Determination of IgE Complexes and of Total IgE by Latex Immunoassay
نویسندگان
چکیده
منابع مشابه
Determination of IgE complexes and of total IgE by latex immunoassay.
A sensitive immunoassay based on latex particle agglutination for the measurement of circulating IgE-containing complexes is described. In this method, the anti-IgE-coated particles are incubated with diluted serum and the resulting agglutination is quantified by turbidimetry or particle counting. In the latter version, the assay is fully automated in a continuous flow system. IgE-containing co...
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Our aims were to evaluate the performance of an automated microfluidic immunoassay system for measuring allergen-specific IgE (sIgE) in sera against an established in vitro assay and to assess the system's diagnostic accuracy against objective clinical criteria for identifying sensitization to specific allergens in daily practice of allergy clinics. Using both the automated microfluidic-based i...
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The prevalence of specific IgE to natural rubber latex proteins in the general population remains uncertain.The purpose of this study was to determine the prevalence of sera containing specific IgE antibodies to latex proteins using immunoenzymatic methods. A population of 500 unselected adult voluntary blood donors was the source of the sera used in this study. Two different immunoenzymatic me...
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Background and aim: Atopic dermatitis (AD) is a chronic relapsing skin disease that commonly begins during infancy and early childhood. Research studies refers to a relationship between exacerbation of atopic dermatitis and exposure to house dust mite (HDM), but the role of HDM in the pathogenesis of AD is controversial. The objecitve of this study was to assess the correlation of density...
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IgE was measured by ELISA in tubes and in microtiter plates, the results being compared with PRIST data. The recommended readings of the tube contents in a spectrophotometer (SPM) were compared with results using a multi-channel photometer (MCP). Geometric mean values (International Units) and standard deviations of 44 normal sera examined by the 3 different methods were: PRIST 16.2 +/- 4.0; SP...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1987
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1987.25.4.245